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Different type of ANA test
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wondergirl47 posted:
I do not understand my test results. Usually ANA is reported as a ratio, like 1:160. My test was reported as 3+. My doctor did not explain this well to me when I questioned him. He just said it's similar to the ratio reporting. That tells me nothing. Anyone know anything about this type of testing?
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lupycakes responded:
Hi,

My ANA tests have always been reported the same way. I believe that's because some labs are starting to use a different method of testing. Here's a link I found that might answer your question. Hopefully Dr. V. Might way in on his thoughts between the two methods.



http://rheumatology.oxfordjournals.org/content/48/8/1013.full



Lisa
 
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wondergirl47 responded:
Thank you for your reply but I am still a bit confused. Is the ELISA method the one expressed as 1:320 or is it the IIF? Which is the newer method--the ratio or the single number? And with the single number, what is the reference range? Any light you can throw on this is greatly appreciated.
 
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lupylisa44 replied to wondergirl47's response:
Can you call your doctor's office and ask them to explain it more clearly? I think it's not asking too much to get an explanation you can understand! Afterall, you are paying them!!! If they give you a hard time, Demand answers!!!

As a lupus patient you MUST BE YOUR OWN ADVOCATE!!! It is probably the single most important thing I have learned in the 29 years I have had lupus.

Lupylisa
With love, with patience and with faith, we'll make our way.
 
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wondergirl47 replied to lupylisa44's response:
Thanks, Lisa. When I called, the Dr. just said the tests are equivalent and didn't elaborate. I think I will have to talk to him about it in person on my next visit. I have not been officially diagnosed with lupus--possible Hashimoto's and a few other issues. Still, do you know if the ratio or the other way of reporting is the newest method?
Thanks again.
 
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lupylisa44 replied to wondergirl47's response:
When I was diagnosed, they used the ratio method and that was a long time ago, so I think the ratio is the older of the two methods. I couldn't find anything online about the "new" method even on the lupus websites they all cite the ratio ANA test,

http://labtestsonline.org/understanding/analytes/ana/tab/test

http://www.lupus.org/webmodules/webarticlesnet/templates/new_empty.aspx?articleid=402

Lupylisa
With love, with patience and with faith, we'll make our way.
 
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lupycakes responded:
Hi,

Sorry I didn't reply sooner - been gone for a couple days... When I saw the rheumy at the Cleveland clinic, I was told the number - not the ratio -was the newer way of testing. My understanding is the newer way might be more accurate as it isn't as susceptible to technician/processing error.



Dr.V ???? Please weigh in with your opinion.

Lisa
 
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wondergirl47 replied to lupylisa44's response:
I have not been able to find anything about the methods other than the ratio either. That is why I posted. I don't even know what the scale is-- if it is 1-3, 1-5, etc. I think I just will have to ask my dr. when I see him, but that is not until October. I just think it is so strange that there is so little information out there about this test method.
 
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lupycakes replied to wondergirl47's response:
Hi,

I just looked at my labs for the range - keep in mind that this range MIGHT be different for different labs, but it would be close:

This is from the Mayo Clinic lab:

< = 1.0 (negative)

1.1 - 2.9 weak positive

> 3.0 positive

Hope this helps!

Lisa
 
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wondergirl47 replied to lupycakes's response:
Thank you Lisa. This help alot. I cannot understand why there is so little information about these new testing methods. Even the Lupus website still refers to the testing in terms of the ratio. As I am just beginning this journey, this is all very confusing to me. I appreciate your taking time to answer my posts. I wish you the best along your journey.
 
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R Swamy Venuturupalli, MD, FACR responded:
There are two methods used to test ANA's. The first method is by immunofluorescence (IIF) which is reported as a ratio. Usually anything over 1:80 is considered a significant result. The second method is by enzyme-linked immunosorbent assay (ELISA) which is reported an absolute number or in the way you mentioned such as 3 . ELISA is increasingly being used because of the ease of testing through automation and no need of a skilled operator in the lab. However, the accuracy of ELISA testing has been questioned. Please see a previous post from me about ANA testing below.

http://forums.webmd.com/3/lupus-exchange/forum/2789


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